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Antibodies certain to the blocking peptide no longer bind on the epitope to the target protein. By evaluating the staining from the blocked antibody vs . the antibody by yourself, you can see which staining is distinct.

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This guide outlines further more feasible optimization of cellular and immunoassay parameters to make sure the best possible final results are...

This guidebook outlines more feasible optimization of cellular and immunoassay parameters to ensure the very best benefits are...

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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Clicking the pictures or hyperlinks will redirect you to definitely a website hosted by BenchSci that gives 3rd-get together scientific content material. Neither the written content nor the BenchSci technological innovation and procedures for choice are evaluated by us; we're furnishing them as-is and with out guarantee of any form, which includes to be used or software from the Thermo Fisher Scientific merchandise introduced.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...

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Blocking peptides are peptides that bind precisely to the target antibody and block antibody binding. These peptide thr77 normally incorporates the epitope regarded because of the antibody.

Target Info Cyclin E belongs for the very conserved cyclin relatives, whose associates are characterized by a remarkable periodicity in protein abundance with the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show distinctive expression and degradation styles which add into the temporal coordination of each mitotic event. Cyclin E kinds a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is degraded as cells progress by way of S section.

Overexpression of this gene continues to be observed in lots of tumors, which ends up in chromosome instability, and so may perhaps contribute to tumorigenesis. This protein was located to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important function in advertising and marketing mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already explained. Two more splice variants were noted but specific nucleotide sequence information just isn't however accessible.

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